Human EJ Antibody / Anti-Glycyl TRNA Synthetase Antibody (EJ/GlyRS) elisa Kit Instruction Manual

**Human EJ Antibody / Anti-Glycyl tRNA Synthetase Antibody (EJ/GlyRS) ELISA Kit – User Manual** **Kit Specification:** This ELISA kit is available in a 48-well or 96-well configuration. It includes the following components: - **Standard Diluent**: 1.5 ml × 1 bottle - **Enzyme Standard Reagent**: 3 ml × 1 bottle (for 48-well) / 6 ml × 1 bottle (for 96-well) - **Standard**: 0.5 ml × 1 bottle, concentration: 2700 ng/L - **Sample Diluent**: 3 ml × 1 bottle (48-well) / 6 ml × 1 bottle (96-well) - **Developer A**: 3 ml × 1 bottle (48-well) / 6 ml × 1 bottle (96-well) - **Chromogen B Solution**: 3 ml × 1 bottle (48-well) / 6 ml × 1 bottle (96-well) - **Washing Solution (20×)**: 20 ml × 20 times × 1 bottle (48-well) / 20 ml × 30 times × 1 bottle (96-well) - **Sealing Film**: 2 pieces (48-well) / 2 pieces (96-well) **Storage Conditions:** - **Kit Storage**: 2–8°C - **Shelf Life**: 6 months from the date of manufacture **Principle of Operation:** The Human EJ Antibody / Anti-Glycyl tRNA Synthetase Antibody (EJ/GlyRS) ELISA Kit utilizes the sandwich immunoassay method. The microplate is pre-coated with a specific antibody against EJ/GlyRS. After incubation with the sample, the target antigen binds to the immobilized antibody. A horseradish peroxidase (HRP)-labeled secondary antibody is then added, forming an antibody-antigen-enzyme complex. Following washing steps, TMB substrate is introduced, producing a blue color that turns yellow upon acid termination. The optical density (OD) at 450 nm is measured, and the concentration of EJ/GlyRS in the sample is determined by comparing it to a standard curve. **Purpose:** This kit is designed for the quantitative detection of Human EJ Antibody / Anti-Glycyl tRNA Synthetase Antibody (EJ/GlyRS) in serum, plasma, urine, cell culture supernatants, and other biological fluids. **Sample Preparation Guidelines:** - **Serum/Plasma**: Centrifuge blood or anticoagulated plasma at 2000–3000 rpm for 20 minutes. - **Urine**: Centrifuge at 2000–3000 rpm for 20 minutes. - **Cell Culture Supernatant**: Centrifuge at 2000–3000 rpm for 20 minutes after collection. - **Tissue Homogenate**: Homogenize tissue in PBS, centrifuge, and collect the supernatant. - **Note**: Avoid repeated freezing and thawing. Store samples at -20°C if not tested immediately. **Operation Steps:** 1. Prepare standard dilutions in a serial manner. 2. Add 40 μL of sample diluent and 10 μL of sample to each well. 3. Seal the plate and incubate at 37°C for 30 minutes. 4. Wash the plate 5 times with diluted washing solution. 5. Add 50 μL of enzyme-labeled reagent to each well. 6. Incubate again at 37°C for 30 minutes. 7. Add 50 μL of TMB A and B, incubate for 15 minutes at 37°C. 8. Stop the reaction with 50 μL stop solution. 9. Measure OD450 using a microplate reader within 15 minutes. 10. Calculate the concentration based on the standard curve. **Notes & Warnings:** - Allow the kit to reach room temperature before use. - Avoid light exposure during TMB development. - Use separate pipettes for each step to prevent cross-contamination. - Do not mix reagents from different batches. - All waste should be handled as biohazardous material. - Always perform a standard curve and replicate measurements for accuracy. **Performance Characteristics:** - Linear range: 0.2 IU/L – 6 IU/L - Correlation coefficient (R²): ≥ 0.95 - Intra-assay CV < 9%, Inter-assay CV < 11% **Technical Support:** Our team provides free technical support during working hours. We also offer sample testing services to help ensure accurate and reliable results. **Important:** This product is for research use only. Not for diagnostic or clinical use.

Black Annealed Wires

Black Annealed Wire (Soft Annealed Wire, Black Iron Wire) offers excellent flexibility and softness, due to its oxygen free annealing process. The black annealed wire is made of SAE 1006, Q195, and the tensile strength is from 300 to 700Mpa and its elongation rate is 10%-25%. The black annealed wire is widely used in construction as tie wire.

Wire Diameter: 0.5mm-5.5mm.

Weight/Coil: 0.1kg-800kg/coil;

Packing: plastic inside and hessian woven outside,or woven bags outside.

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