Guangrui Bio, a trusted supplier of high-quality ELISA kits in China, specializes in providing reliable reagents for scientific research. Our product line includes ELISA kits, biological reagents, biochemical reagents, antibodies, culture media, and standard reference materials. We offer competitive pricing on our ELISA kits and are committed to delivering accurate and consistent results for your experiments. Whether you're conducting clinical testing or basic research, our products are designed to meet the highest standards.
When it comes to ELISA testing, proper sample collection and preparation are crucial for reliable outcomes. Common samples used in clinical testing include blood (venous blood), urine, feces, cerebrospinal fluid, pleural and ascitic fluids, prostatic fluid, semen, and vaginal secretions. Each type of sample has specific collection, handling, and Storage requirements.
For blood samples, several physiological factors can influence test results, such as smoking, eating, physical activity, emotional stress, pregnancy, body position, and even time of day. To ensure consistency, it's best to collect samples under standardized conditions. If unavoidable, these factors should be clearly noted on the sample.
Peripheral blood is typically collected from the inner side of the left ring finger, ensuring that the area is free from frostbite, inflammation, swelling, or damage. If the selected site is not suitable, another finger can be used. For burn patients, blood can be drawn from intact skin areas. For certain tests like white blood cell counts, maintaining consistent conditions is essential to avoid large fluctuations caused by external factors.
Venous blood is often used for coagulation tests, such as platelet count, bleeding time, and clotting time. It's important to know whether the patient has taken anticoagulant or procoagulant medications, as these can significantly affect the results. Most ELISA tests use serum, but some require plasma, especially for coagulation-related analyses.
Some tests, like glucose and lipid levels, are highly sensitive to dietary intake and circadian rhythms, so fasting blood samples are usually preferred. Others, such as serum enzymes (e.g., ACP activity), may lose activity when stored at 0–4°C, requiring rapid processing. Additionally, factors like exercise can greatly impact enzyme levels, such as creatine kinase. Hemolysis during blood collection must also be avoided, particularly when measuring serum potassium or LDH levels.
At Guangrui Bio, we understand the importance of precise sample handling and provide detailed guidelines to help you achieve accurate and reproducible ELISA results. Contact us today to learn more about our ELISA kits and other research reagents. Let us support your scientific success with quality, reliability, and expert service.
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