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Abstract: A method for the direct separation and determination of ccfâº, Cuâº, Ni²âº, Zrâ´âº and ten other metal ions in water samples using capillary electrophoresis was developed. The influence of buffer type, concentration, pH, and organic modifiers on the separation efficiency was systematically investigated. After optimization, the separation conditions were set as follows: a 150 mM HAc-NaAc (20%) buffer solution with a pH of 4.30, an applied voltage of 25 kV, and a detection wavelength of 223 nm. Under these conditions, the complexes formed by Ni²⺠and Cu⺠with o-phenanthroline were well separated within 4 minutes. The relative standard deviations for migration time and peak area were below 0.95% and 3.5%, respectively, indicating excellent repeatability and precision. A good linear relationship between concentration and peak area was observed over a wide range, confirming the reliability and accuracy of the method for quantitative analysis. This approach offers a rapid, efficient, and sensitive means for the simultaneous determination of multiple metal ions in environmental water samples.
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