![<?echo $_SERVER['SERVER_NAME'];?>](/template/twentyseventeen/skin/images/header.jpg)
The competition inhibition method relies on the interaction between the enzyme-labeled antibody and the sample antibody in a shared system, where both compete for binding to the solid-phase antigen. According to theory, the sample should be mixed with the enzyme-labeled antibody first before adding the reaction reagents. However, in practice, these components are often added separately into the reaction wells, which can disrupt the balanced competition and lead to deviations from the expected proportional relationship. This issue becomes more pronounced under conditions of extended incubation times or elevated temperatures, significantly affecting the accuracy of the results.
Changhai Hospital at the Second Military Medical University conducted a study to evaluate this effect. In their experiment, 94 negative specimens were diluted 1:30 with physiological saline. After the specimens were added to the wells, the enzyme-labeled antibodies were introduced at different time intervals. The results showed how varying the timing of antibody addition impacted the outcomes:
| Placement Time (min) | Number of Negative Specimens | Threshold - A450nm Value | False Positive Rate (%) |
|----------------------|------------------------------|---------------------------|--------------------------|
| <0.3 | 75 | 10 | 0 |
| 0.3~0.7 | 10 | 3 | 6 |
| >0.7 | 3 | 6 | 0 |
| 2 | 68 | 13 | 7.4 |
| 5 | 65 | 15 | 8 |
| 6 | 6 | 7.4 | 10.6 |
| 10 | 56 | 16 | 12 |
| 20 | 38 | 18 | 17 |
| 30 | 21 | 12 | 18 |
| 43 | 57.4 | | |
As shown in the table, when the specimen and enzyme-labeled antibody were added simultaneously, no false positives were observed. However, when the enzyme-labeled antibody was added after a two-minute delay, a 7.4% false positive rate occurred. After 30 minutes, the false positive rate increased dramatically to 57.4%. These findings strongly suggest that for optimal results, the enzyme-labeled antibody should be added immediately after the sample to maintain the integrity of the competitive binding process.
Shanghai Hengyuan offers a wide range of products and services, including ELISA kits, immunohistochemistry kits, radioimmunoassay kits, standards, sera, antibodies, culture media, cells, biological reagents, and laboratory consumables. We guarantee quality, and any quality issues will be resolved with free replacements. Additionally, we provide comprehensive technical support and free testing services. Every product comes with full after-sales service, and our team is committed to resolving any customer inquiries within one working day.
Microfiber Flat Mops all produced by 80% poyester and 20% polyamide microfiber mop fabrics.With finished style with banding edge and stitching edge,and very easy to attached with mop frmae with loop and hook velcro.Washable more than hundreds times.Commercial than tradional cotton mop.You can clean al kinds of floor surface with only water.
Microfiber Flat Mop,Flat Squeeze Mop,Flat Mop Pads,Flat Squeeze Mop And Bucket
jiangsu qiyun cleaning knitting product co.,ltd , https://www.jarfrry.com