Human β2-GP1 ELISA Kit

**Human β2-GP1 ELISA Kit – For the quantitative in vitro determination of Human β2 glycoprotein 1 concentrations in serum, plasma, cerebrospinal fluid, tissue homogenate, and other body fluids. Intended for laboratory research use only. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE. Please read this entire package insert before using the kit.** **INTENDED USE AND TEST PRINCIPLE** This β2-GP1 ELISA Kit is designed for research purposes only and is not intended for diagnostic or therapeutic procedures. The assay is based on a competitive enzyme-linked immunosorbent assay (ELISA) format. The color change from blue to yellow occurs upon addition of the Stop Solution, and the optical density (OD) is measured at 450 nm using a microplate reader. A set of calibration standards is included to generate a standard curve, which allows for accurate quantification of β2-GP1 in unknown samples by comparing their OD values. **SAMPLE COLLECTION AND STORAGE** - **Serum**: Use a serum separator tube. Allow samples to clot for 2 hours at room temperature or overnight at 4°C before centrifugation at 2000×g for 20 minutes. Remove serum and analyze immediately or aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. - **Plasma**: Collect using heparin as an anticoagulant. Centrifuge within 30 minutes of collection at 2000×g for 30 minutes at 2–8°C. Store at -20°C. - **Cell culture supernatants, tissue homogenates, and other biological fluids**: Centrifuge to remove particulates and analyze immediately or store at -20°C. Ensure samples are properly centrifuged and free from hemolysis or debris. **MATERIALS REQUIRED BUT NOT SUPPLIED** - 37°C incubator - Microplate reader capable of measuring absorbance at 450 nm - Precision pipettes, disposable tips, and absorbent paper - Distilled or deionized water **REAGENTS PROVIDED** All reagents should be stored at 2–8°C. Check expiration date on the label. - Microtiter strip plate (12×8 or 12×4 strips) - Standard (6 vials, 0.5 ml/vial) - Sample Diluent (6.0 ml or 3.0 ml) - HRP-Conjugate Reagent (10.0 ml or 5.0 ml) - 20X Wash Solution (25 ml or 15 ml) - Chromogen Solution A (6.0 ml or 3.0 ml) - Chromogen Solution B (6.0 ml or 3.0 ml) - Stop Solution (6.0 ml or 3.0 ml) - Closure Plate Membrane (2 units) - User Manual (1 copy) - Sealed Bags (1 unit) **NOTES** - Standard concentrations: 16, 8, 4, 2, 1, 0.5 ng/ml. - If sample values exceed the highest standard, dilute with Sample Diluent. - Ensure all reagents are at room temperature (20–25°C) before use. - Do not use water baths to thaw samples or reagents. - Never use reagents past their expiration date. - Only use deionized or distilled water for dilutions. - Keep unused microtiter plate strips in sealed bags with desiccant. - Use fresh pipette tips for each transfer to prevent contamination. - Handle all blood-derived products as potentially infectious. - Dispose of all samples according to local biohazard regulations. - Allow liquid to stand for at least 30 minutes before disposal to inactivate viruses. - Substrate solutions are sensitive; avoid contamination. - Store the kit at 2–8°C for frequent use or -20°C for long-term storage. **ASSAY PROCEDURE** 1. Prepare all reagents and microtiter plate. 2. Add 50 µl of standard or sample to appropriate wells (except blank). Cover with adhesive strip and incubate for 60 minutes at 37°C. 3. Wash plate 4 times using 1X Wash Solution (manual or automated). 4. Add 50 µl of Chromogen A and 50 µl of Chromogen B to each well. Incubate for 15 minutes at 37°C, protected from light. 5. Add 50 µl of Stop Solution to each well. Read OD at 450 nm. 6. Plot average OD values against standard concentrations to create a standard curve. Calculate sample concentrations by interpolation. **PERFORMANCE CHARACTERISTICS** - Intra-assay CV <15%, Inter-assay CV <15% - Assay range: 0.5 ng/mL – 16 ng/mL - Sensitivity: <0.1 ng/mL - Cross-reactivity: No significant cross-reactivity with other proteins - Storage: 2–8°C (frequent use); 6 months at -20°C **IMPORTANT REMINDER** Always follow good laboratory practices. Ensure all steps are performed accurately and consistently to obtain reliable results. Each user should prepare their own standard curve for best performance.

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