TetraOneTM KO - a major breakthrough in gene knockout technology

Recently, Cyegen Biosciences announced the launch of its global patented technology TetraOneTM KO, which is not only comparable in speed to TALEN, CRISPR/Cas9 (the custom cycle of knocking/knocking ES target genes into mice is reduced to 6 months) ), and avoid the revolutionary technology that TALEN, CRISPR/Cas9 off-target effects. The emergence of TetraOneTM KO is a major breakthrough in the preparation of knockout mice by ES targeting technology.

Technical progress in genome editing

Traditional ES targeting, TALEN and CRISPR/Cas9 are the three technologies and main tools for genome editing. A new generation of nuclease gene editing technology TALEN and CRISPR/Cas9 as an innovation in modern molecular biology technology, it combines the advantages of simple and rapid transgenic technology, no species restriction and precise precision of ES cell gene targeting technology, in just a few years Time is favored by the majority of researchers. However, because its off-target effect has not been avoided so far, and it is difficult to master the gene knock-in of large fragments, it is still far from mature. How to improve the efficiency of TALEN and CRISPR/Cas9 and reduce the off-target effect will be the main research direction in the future.

ES targeting based on homologous recombination is the industry gold standard for gene knockout. It integrates foreign genes into a certain site on the target cell genome by homologous recombination technology to achieve a fixed-point modification of a certain gene in the genome. purpose. Gene targeting technology is now widely recognized as an ideal method for specific modification and transformation of genetic material in living organisms. In particular, the establishment of conditional and inducible gene targeting systems makes the target modification of genes in time and space more clear and more accurate and reliable. Still, the lengthy build cycle (which takes 8-12 months) has discouraged many researchers.

It is clear that TALEN, CIRSPR/Cas9 and traditional ES targeting gene knockout technologies have complementary roles, while TetraOneTM KO technology is a combination of both. This is undoubtedly the most important revolutionary technology in the current research of genome editing. It makes it possible to build gene-targeted mice more quickly and accurately.

The technical principle of TetraOneTMKO

Saiye Biotechnology has established a genetically superior TetraOneTM ES cell line using unique architecture and genetic modification technology. By microinjection in the early stage of embryonic development, TetraOneTM ES cells are 100% replaced by endogenous ES cells. The "Synthesis" phase provides direct access to the patented technology for gene targeting in TetraOneTM mice. The results of the experiment showed that the characteristics of TetraOneTM mice were consistent with those of F1 mice produced by traditional ES targeting techniques.

TetraOneTMKO service provided by Saiye Bio

Saiye Biotech provides technical services for complete knockout mice, conditional knockout mice, and targeted knock-in mice constructed using TetraOneTM KO technology. The build process directly spans the chimera phase, achieving a very fast cycle of 6 months to complete the build:

About Saiye Bio

Saiye Biotech Group is the world's leading diversified multinational biotechnology entity. As the world's largest commercial technology platform for transgenic/knockout mode animals, the Safran Model Animal Center has an animal population of more than 100,000, which can be used to construct gene knockouts every year. There were 500 cases of mouse model and 5000 cases of transgenic mouse model, and the number of citations has reached 600. At present, it has served hundreds of top life science laboratories and multinational pharmaceutical companies around the world. It is the most popular and recognized Chinese genetic engineering model animal business service platform.

In 2009, the company passed the ISO9001:2008 certification and passed the review in 2012 to ensure that all products and technical services provided to experts and scholars meet international standards. The successful passing of AAALAC certification has made Saiye one of the few units in South China that has obtained international certification from the International Laboratory Animal Evaluation and Accreditation Committee. This is not only a symbol of the quality of experimental animals and biosafety standards, but also a quality mark for international cutting-edge medical research.

Saiye Company has established a living laboratory test and isolation center in Santa Clara, California, USA. It has become the only bio-investment company with the ability to export live-type experimental rodents in China; it has become the first in South China through the assessment of the national entry-exit inspection and quarantine department. The pilot unit for entry and exit of biological materials in green channels has accelerated the process of approval of entry and exit of experimental animals and further enhanced the ability of the international service of the Animal Model of the competition model.

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