Progress in three-dimensional electron microscopy

Progress in 3D Electron Microscopy Fan Jingwei Abstract: The method of recombining the three-dimensional structure from the electron microscopy image of biological samples (cells or macromolecules) has made significant progress in recent years. This is the cryo-electron microscopy sample preparation technology, electron microscope equipment, image processing and analysis methods. The combined results of several aspects of progress. The advancement and improvement of the three-dimensional electron microscopy method enables cell biologists to understand the spatial relationships between various related organelles in complex cellular processes, so that molecular biologists can not only study samples that can form two-dimensional crystals, and It provides a powerful means for the analysis of structures of macromolecules or molecular aggregates that have important biological functions but cannot form two-dimensional crystals.


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