Technical principle and progress of Shanghai Haozhuang solid phase extraction plant

Shanghai Haozhuang Instrument Co., Ltd. has developed the LNB brand solid phase extraction device, which is energy-saving and environmentally friendly, leading the country. Professional manufacturing is more secure and affordable

The principle of solid phase extraction: solid phase extraction is a physical extraction process including liquid phase and solid phase. In solid phase extraction, the adsorption force of solid to the isolate is greater than the solvent that dissolves the isolate. When the sample solution passes through the adsorbent bed, the isolate is concentrated on its surface, and other sample components pass through the adsorbent bed; by adsorbing only the isolate and not adsorbing other sample components, a high-purity and concentrated isolate can be obtained.

1. Retention and elution: The most common method in solid phase extraction is to install the solid adsorbent in a syringe column and pass the sample solution through the adsorbent bed. The compounds in the sample either pass through the adsorbent or remain in the adsorption On the agent (depending on the relative adsorption of the adsorbent to the solvent). "Retention" is a phenomenon that exists between the molecules of the adsorbent and the separator, causing the separator to not move on the adsorbent when the sample solution passes through the adsorbent bed. Retention is the effect of three factors: isolate, solvent and adsorbent. Therefore, the retention behavior of a given isolate varies in the presence of different solvents and adsorbents. "Elution" is a process in which the separator remaining on the adsorbent is removed from the adsorbent by adding a solvent that attracts the separator more strongly than the adsorbent.

2. Capacity and selectivity: The capacity of the adsorbent is under the optimal conditions, and the unit amount of adsorbent can retain a total amount of strongly retained isolate. The capacity of different bonded silica adsorbents varies widely. Selectivity is the ability of the adsorbent to distinguish the isolate from other sample matrix compounds, that is, to retain the isolate to remove other sample compounds. A highly selective adsorbent is one that retains only the isolate from the sample matrix. Adsorbent selectivity is the function of three parameters: the chemical structure of the isolate, the nature of the adsorbent, and the composition of the sample matrix.

3. The brief process of solid phase extraction: A sample includes the separator and interfering substances through the adsorbent; the adsorbent selectively retains the separating substances and some interfering substances, and the other interfering substances pass through the adsorbent; The interferences previously retained are selectively rinsed away, and the isolates remain on the adsorbent bed; the purified and concentrated isolates are eluted from the adsorbent.

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