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First, the question is raised:
When it comes to microbial colony counting—whether using the pour plate method, spread plate method, spiral plating, or membrane filtration, along with 3M test plates—each technique has its own unique characteristics. Traditional automatic colony counters often require users to adjust specific parameters in order to achieve the best results. However, most users lack a deep understanding of these settings and how they should be applied under different conditions. This leads to confusion and improper use, which can cause the automatic colony counter to become nothing more than an expensive display.
This issue is quite common, as confirmed by small-scale surveys among users. The problem isn’t necessarily with the technology itself, but rather with the user’s lack of proper training or guidance. In many cases, even experienced engineers may not fully understand how to configure the system for optimal performance, resulting in subpar outcomes.
Wanshen Detection Technology has recognized this critical challenge and successfully developed an innovative fifth-generation solution that enables foolproof, one-button automatic colony counting for up to six plates at once. Much like the definition of a fifth-generation fighter jet, this new generation of colony counter is built on intelligence and automation. To validate the effectiveness of this breakthrough, Wanshen tested the system using mobile phone photos taken in normal ambient lighting conditions. The evaluation included various methods such as pour plate, spread plate, spiral plating, membrane filtration, and 3M test plates, featuring different types of colonies, including flower-shaped molds, as well as media with grid lines or colored surfaces.
Second, the specific solution:
The innovative fifth-generation technology of Wanshen HiCC series automatic colony counting software is reflected in five key aspects:
1. Intelligent Colony Automatic Enhancement Technology. Distinguishing between colony targets and non-colony backgrounds is crucial for efficient and accurate automatic counting. Creating a uniform lighting environment is one way to achieve this. For example, the illumination uniformity of scanning imaging is significantly higher than that of traditional light boxes used in home or laboratory settings. This makes scanning imaging more suitable for one-button automatic counting. Additionally, the system automatically corrects inconsistencies in the medium or changes in ambient lighting, ensuring reliable results. Figures 1 and 2 show the effectiveness of this technology in correcting mobile phone photographs taken under normal lighting conditions.
2. Fast Level Set Active Contour Extraction Technology. In complex growth scenarios, the color difference between colonies and the background may be minimal, making it difficult for standard segmentation techniques to identify them. Wanshen's fast level set active contour extraction technology efficiently identifies and extracts even the most challenging colonies at high speed. It also enables automatic classification and counting based on color and size, while recording user actions for batch processing. This allows for a near-foolproof automatic counting experience. Figures 3 and 4 show the results of one-button counting and marking performed by the Wanshen system on mobile phone images.
3. No-Trace Elimination of Grids and Text. Grid lines or text on paper media or filters can interfere with colony detection. Wanshen's seamless elimination technology removes these interferences without leaving any traces, as shown in Figure 4B.
4. Automatic Segmentation and Counting of Bacillus Microscopic Morphology. Traditional counting methods rely on circular segmentation, which is not effective for long rod-shaped bacteria under a microscope. Wanshen Smartdownâ„¢ technology accurately counts both round colonies and bacillary forms. The results of automatic counting of Gram-negative bacilli are shown in Figure 5 below.
5. Auto-Focus High-Resolution Colony Imaging. Whether using a scanner, Canon EOS SLR camera, or mobile phone, the system eliminates the need for manual focusing to capture clear images. The imaging quality achieved by the Wanshen system using these devices is demonstrated below.
Third, the video demonstration of the highly complex automatic colony counting process:
To help you better understand Wanshen's advanced technology, here are two sample videos:
1) Wanshen HiCC automatic counting video of cell phone photo culture colonies: Watch Here
2) Wanshen HiCC automatic counting video of cell phone photo shoots: Watch Here
All manufacturers are welcome to submit their images for technology comparison, and all users are encouraged to send their samples for real-world evaluation.
Fourth, the outlook:
From the Wanshen HiCC (fifth generation) automatic colony counting technology, it is clear that even mobile phone photos can achieve fast and accurate colony counting. This shows that Wanshen HiCC technology has significantly reduced the environmental requirements for colony imaging, paving the way for a future where low-cost, accurate automatic colony counting without the need for specialized imaging equipment becomes widely accessible.
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